Figure 1. Ro3206145 inhibits p38 MAPK activity ex vivo.
(A) Example western blot of phosphorylated activating transcription factor 2 (p-ATF2) following a kinase reaction with phosphorylated p38 MAPK that was immuno-precipitated from rat retinal explants. Explants were exposed to ultra-violet (UV) light to activate p38 MAPK. Increasing concentrations of Ro3206145 were effective at inhibiting p38 MAPK activation of ATF2. (B) Densitometer quantification (of the ATF2 assay (n≥3 for each condition) shows significant increase in p38 MAPK activity with UV exposure relative to naïve controls (* p = 0.01). Ro3206145 (in DMSO) reduces activity, reaching significance compared to UV exposure alone for 10 μM and higher (** p ≤ 0.003).