Figure 3. IL-10 competence is independent of B cell precursor populations and IL-10.
(A) IL-10 competence is independent of precursor B cell origin. Bone marrow and fetal liver hematopoietic cells from congenic CD45.1 transgenic donor mice were transferred i.v. into recipient Rag2−/− mice. Six weeks later, CD45.1+CD19+ B10 cells within the peritoneal cavity and spleen of recipient mice were quantified. Bar graphs show mean (±SEM) B10 cell frequencies and numbers from four recipient Rag2−/− mice in two experiments. Significant differences between sample means are indicated: * p<0.05. (B) Peritoneal cavity B cell subsets develop normally in IL-10−/− mice. B cells isolated from 10-12 week-old wild type or IL-10−/− littermates were stimulated with L+PIM for 5 h before immunofluorescence staining with flow cytometry analysis. Bar graphs indicate mean (±SEM) frequencies or numbers of B cells with B1a, B1b or B2 phenotypes (n=8 mice in two experiments). (C) IL-10 is not required for peritoneal cavity B10 cell development in 10BiT mice. B cells isolated from 10-12 week-old 10BiT or IL-10−/−10BiT littermates were stimulated with L+PIM for 5 h before immunofluorescence staining with flow cytometry analysis. Bar graphs indicate mean (±SEM) frequencies or numbers of IL-10+ cells among B cells with B1a, B1b or B2 phenotypes (n=3-4 mice/group).