Fig. 3.
Transcriptional and epigenetic regulation of the Srebp2 gene. A: A schematic diagram of the proximal promoter of the mouse Srebp2 gene, indicating two putative IRE (IRE1 and IRE2) and a sterol-response element (SRE). The arrow denotes the transcription start site. The conservation of IRE1 sequences among mouse, rat, and human Srebp2 genes is also shown. B–D: Luciferase reporter assays for WT and IRE-mutant Srebp2 promoters were performed in HEK293 cells that were transfected with respective promoter constructs with vector GFP, FoxO1, or FoxO3. E: ChIP analysis of FoxO3 association with the mouse Srebp2 gene promoter and histone H3 acetylation in the proximal IRE1 region in mouse primary hepatocytes transduced with GFP (AdGFP) and FoxO3 (AdFoxo3) adenoviruses. DNA copy numbers were normalized to Ppia ChIP or total histone H3 ChIP. Data are mean ± SEM; *P ≤ 0.05 by t-test.