FIG. 2.
The effect of Fibrin Hydrogels (FH) on Par-C10 and PG single cells. Single cells grown on FH for 3 days were analyzed using immunofluorescence microscopy using rabbit anti-ZO-1 (A, D, E, H; green), phalloidin staining (B, D, F, H; red), and Hoechst nuclear stain (C, D, G, H; blue). The xy cross-section images were obtained using a Carl Zeiss 510 confocal microscope. All scale bars represent 50 μm. Cells were stimulated with Carbachol (100 μM) and changes in the Fura-2 Fluorescence ratio recorded at 340 and 380 nm were monitored (I) as described in Materials and Methods. Results from a representative of three experiments are shown. Color images available online at www.liebertpub.com/tea