Figure 3.
(A) TLR4 deficient mice C3H/HeJ mice express lower levels of (i) IL-12p35 (p<0.01), and higher levels of (ii) IL-12p40 (p<0.05) as well as (iii) IL-23p19 (p<0.01) in their tumors compared to C3H HeN mice as determined by quantitative real-time PCR. (B) Mice were fed once with DMBA by oral gavage, and on day 6 mice were sacrificed and spleens were removed. T cells, or CD11c+ cells were purified from spleen as described in the Methods section. T cells were incubated with CD11c+ cells for 72h and IFN-γ and IL-17 levels were determined in the supernatant by ELISA. (i) Addition of anti-IL-12p70 antibody to cultures significantly inhibits the production of IFN-γ (p<0.05), and (ii) addition of anti-Il-23p19 antibody to cultures significantly inhibits the production of IL-17 (p<0.05).