. Author manuscript; available in PMC: 2014 Sep 1.

Published in final edited form as: J Immunol. 2013 Jul 26;191(5):2126–2133. doi: 10.4049/jimmunol.1301092

Table II.

Serological reactivity of PBC to the E2 lipoyl domain mutant constructs.

Mutant No.	PBC Sera^a		Purified PBC IgG^b
Mutant No.	IgG	IgM	Purified PBC IgG^b
WT	1	1	1
PM 1	0.891±0.014	0.741±0.021	0.938±0.084
PM 2	0.968±0.029	0.804±0.032	0.874±0.057
PM 3	1.013±0.025	1.310±0.066	0.807±0.044
PM 4	0.476±0.029^***	0.504±0.043^**	0.408±0.052^***
PM 5	0.944±0.031	1.220±0.073	0.797±0.066
PM 6	0.990±0.022	1.233±0.063	0.888±0.031
PM 7	1.084±0.024	1.768±0.141^***	0.848±0.029
PM 8	1.011±0.022	1.542±0.103^***	0.876±0.019
PM 9	1.081±0.018	1.129±0.052	1.088±0.030
PM 10	0.706±0.029^***	0.781±0.054	0.552±0.065^***
PM 11	1.066±0.021	1.096±0.067	0.700±0.065^**
PM 12	0.861±0.023	0.823±0.069	0.729±0.055^*
PM 13	0.659±0.034^***	0.768±0.096	0.482±0.074^**
PM 14	0.962±0.022	0.876±0.069	0.848±0.053
PM 15	1.081±0.019	1.062±0.060	0.858±0.045
PM 16	1.175±0.025	1.426±0.124^**	1.022±0.039
PM 17	1.126±0.019	1.357±0.076^***	0.851±0.088
PDM 1	0.334±0.029^***	0.253±0.034^***	0.075±0.023^***
PDM 2	0.461±0.031^***	0.435±0.045^***	0.663±0.069^***
PDM 3	0.066±0.009^***	0.093±0.016^***	0.024±0.007^***
PDM 4	0.111±0.017^***	0.095±0.016^***	0.043±0.016^***
PTM 1	0.017±0.004^***	0.044±0.009^***	0.038±0.017^***
PTM 2	0.019±0.003^***	0.054±0.012^***	0.050±0.007^***
PQM	0.024±0.005^***	0.066±0.012^***	0.075±0.031^***

Relative ratio of IgG and IgM reactivity to wild type determined by ELISA at 1:4000 sera dilution (n=60).

Relative ratio of purified IgG reactivity to wild type determined by ELISA (n=10).

Asterisks indicate significant differences between mutant protein and wild type protein (*, p < 0.05; **, p < 0.01; ***, p < 0.001; two-tailed unpaired t-test with Welch’s correction).