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KNH1144 SOSIP.681 gp140 aggregates can be dissociated by nonionic detergents. SEC-fractionated gp140 trimers were incubated with the indicated concentrations (% wt/vol) of n-dodecyl-β-d-maltopyranoside (DDM) and before analysis by BN-PAGE followed by Coomassie blue staining. The positions where aggregates and trimers migrate are indicated, as are the molecular masses (kilodaltons) of marker proteins (left lane).
