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Krebs Henseleit buffer preparation
| Ingredients | Concentration |
|---|---|
| NaCl | 60 g/l |
| KCl | 1.75 g/l |
| KH2PO4 | 1.6 g/l |
| N-Acetyl-l-cysteine | 5 mM |
| EGTA | 2.4 M |
The following reagents are dissolved in distilled water and then the pH is adjusted to 7.4. The buffer is sterile filtered and stored at 4 °C
