Table 11.
Perfusion solution II preparation: Solutions A and B are prepared separately
| Additive | Concentration |
|---|---|
| Solution A | |
| NaCl | 67 mM |
| KCl | 6.7 mM |
| Hepes | 100 mM |
| Albumin | 0.5 % (w/v) |
| Solution B | |
| CaCl2 | 4.8 mM |
A volume of 1.3 l of solution A and 150 ml of solution B are mixed and made up to 1.5 l with distilled water. The pH is then adjusted to 7.5. The buffer is sterile filtered and stored at 4 °C. If the solution is not prepared freshly, re-check the pH value at 37 °C immediately before the isolation procedure and adjust if necessary