Figure 8. Lupus nephritis in Scarf1−/− mice.
a, Cryosections of kidneys from 24 week-old Scarf1+/+ and Scarf1−/− female mice were fixed with 4% paraformaldehyde and stained with periodic acid-Schiff (PAS, 12 mice per group). b, Deposition of immune complexes in glomeruli. Cryosections of kidneys from 24 week-old Scarf1+/+ and Scarf1−/− female mice were stained with AlexaFluor 488-conjugated antibody to mouse IgG (green) and nuclear stain DAPI (blue), and analyzed by fluorescent microscopy. c, Incidence and severity of renal disease in Scarf1−/− mice (24 mice per group). d, e, f, Scarf1+/+ and Scarf1−/− mice were placed in a metabolic chamber for 24 hours and the urine collected was analyzed for proteinuria, percentage of blood, and leukocytes in the urine of mice were determined by Roche Chemstrip 5OB for urinalysis (12 mice per group). g, Blood urea nitrogen levels in Scarf1+/+ and Scarf1−/− mice. Data represent average of three independent experiments (n=10 mice), error bars denote the means and s.d. of at least triplicate measurements. None detected (ND). *P<0.01, ** P<0.001; Student’s t test.