FIGURE 6.
Immunization with human CD4–CD8 lipopeptides protects against HSV-1 replication in the eyes of HLA Tg rabbits. HLA Tg rabbits were immunized with the three human CD4–CD8 lipopeptides (four in experiment 1, four in experiment 2) or mock-immunized (two in experiment 1, two in experiment 2). Two weeks after the third immunization, all rabbits were ocularly challenged with 2 × 105 PFU per eye of HSV-1. Tears were collected daily from all eyes (20 eyes per group) on the days indicated, and the amount of infectious virus was determined by standard plaque assays. The p value is calculated using data from days 4 and 5 analyzed by ANOVA followed by Dunnett’s post test to identify differences between groups.