Figure 2. UNC-13L and UNC-13S both reconstitute locomotion behavior in unc-13 mutants.
(A and B) Locomotion behavior was analyzed for the indicated genotypes. (A) Representative 20 s locomotion trajectories are shown (n = 10 animals for each genotype). The starting points for each trajectory were aligned for clarity. (B) Locomotion rates are compared. UNC-13L (KP6893) and UNC-13S (KP6899) refer to unc-13(s69) mutants containing the indicated transgenes. (C and D) Endogenous EPSCs were recorded from adult body wall muscles of the indicated genotypes. Representative traces (C) and summary data (D) are shown. Values that differ significantly from wild-type controls are indicated (***p<0.001, *p<0.05). The number of animals analyzed is indicated for each genotype. Error bars indicate SEM.
Figure 2—figure supplement 1. UNC-13L and UNC-13S both restore SV priming.
Sucrose-evoked EPSCs were recorded from adult body wall muscles of the indicated genotypes. Representative traces (A) and summary data (B) are shown. The bar above each trace indicates the 1 s application of sucrose. (B) The total synaptic charge transfer was measured for 1 s after initiation of the sucrose stimulus for each genotype. UNC-13L and UNC-13S refer to unc-13(s69) mutants containing the indicated transgene. Values that differ significantly from wild-type controls are indicated (***p<0.001, **p<0.01). The number of animals analyzed is indicated for each genotype. Error bars indicate SEM.