Figure 2.
MTBP inhibits cell migratory potential independently of MDM2 and p53. (a) MDM2−/−p53−/− MEFs were transfected with non-target (grey, cont) or MTBP-specific (black, MTBP) siRNAs. (b) SaOs2-LM7 and U2OS cells were infected with empty (grey, cont) or MTBP-encoding (black, MTBP) adenoviruses (Adeno). (c) SaOs2-LM7 and U2OS cells were transfected with non-target (grey, cont) or MTBP-specific (black, MTBP) siRNAs. Thirty six (36) hours after manipulation of MTBP expression, cells were examined for migratory potential. Migrating cells were stained and entire fields were counted. Representative images are shown below the graphs. Graphs showing relative cell migration (%) compared to the number of migrating cells in control. Western blotting results below the graphs showing successful manipulation of MTBP expression. Error bars are means ± S.D. from three independent experiments. *, P < 0.05 and **, P < 0.01; t test.