Figure 3. Analysis of mice with epidermal-specific deletion of Lkb1.
A) Gross image of K14:Lkb1- and litter mate control mice at 1 wk (upper panel) and Northern blot showing efficient deletion of Lkb1 in K14:Lkb1- keratinocytes compared to keratinocytes from littermate controls (lower panel); B i) Histological analysis of K14:Lkb1- and litter mate control dorsal skin. Epidermis from K14:Lkb1- and wild type mice show comparable staining for the differentiation markers, ii) Keratin 14, iii) Keratin 1 and iv) involucrin and comparable rates of BrdU staining (v). The immunofluorescence staining is green. DAPI stained nuclei are pseudo-colored red. C) Survival analysis of the DMBA-treated cohorts documenting mortality due to SCC in K14:Lkb1-1 mice and littermate control mice; D) Histology of SCC from a DMBA-treated K14:Lkb1-1 mouse.