Fig. 1.
Reduced tonic current in α5-/- hippocampal slice and cultured neurons. (A) BIC (100 μM; solid bar) inhibited a tonic current recorded from CA1 pyramidal neurons in hippocampal slices from WT but not α5-/- mice. (B) Scatterplots illustrate the amplitude of the tonic current in hippocampal slices. The horizontal lines in B and D represent the mean amplitude of the tonic current. (C) Tonic current is reduced in α5-/- neurons grown in primary cultures. (D) Scatterplots illustrate the amplitude of the tonic current in cultured neurons. Inset shows that mIPSCs in α5-/- and WT neurons are similar. (E and F) The all-point histograms illustrate the amplitude of the current in the absence (black) and presence (gray) of BIC for WT and α5-/- cultured neurons. The dashed line represents the line of best fit.