Figure 5. Depletion of the H3K9 HMTases but not of Cbx3 induces a change in PTMs on the histone H3 K9/K14 peptide in pre-iPSCs.
A) Percentages of the different isoforms of the H3 peptide containing the amino acids 9 to 17 (in which K9 and K14 are modified) in pre-iPSCs, three days post-transfection of control and 3XHMT siRNAs, determined by qMS. The data for two biological replicates are given. Source data are provided in Supp Table 2.
B) As in (A), except that the qMS approach was performed upon Cbx3 knockdown.