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. Author manuscript; available in PMC: 2013 Jul 26.
Published in final edited form as: J Vasc Res. 2008 May 31;46(1):15–24. doi: 10.1159/000135661

Figure 2. A10 cells transport ascorbate by both the SVCT1 and SVCT2.

Figure 2

Panel A: Rinsed cells were incubated in KRH at 23 °C in the presence of 5 mM D-glucose, 0.5 mM GSH, and 0.05 µCi of [1-14C]ascorbate in the absence of further treatments (Con), or in the presence of 25 µM cytochalasin B (CB), 1 mM ascorbate, 40 mM 3-O-methylglucose (MG), KRH in which sodium chloride was replaced with 140 mM choline chloride (Na (-)), or 1 mM sulfinpyrazone (SULF). After 30 min, the cells were rinsed 3 times in KRH and taken for assay of ascorbate transport. Results are shown from 4 experiments, with an asterisk (*) indicating p < 0.05 compared to the control sample. Panel B: Immunoblotting of the SVCT2 transporter.