Figure 2. Primary candidates were validated in vivo.
A) The number of hit candidates that validated (va) or did not validate (nv) in vivo is shown. Genes that upon knockdown caused severe developmental defects and therefore could not be assayed are also indicated (dd). A full list of validated fly lines and corresponding transposon derepression information is available in Table S3.
B) Validated hits are preferentially expressed in ovaries. The percentage of genes that are enriched in ovaries compared to whole fly is shown for the three categories. This data is based on mRNA signals on Affymetrix expression arrays available from FlyAtlas (Chintapalli et al., 2007).
C) The fraction of genes causing sterility upon knockdown is shown. Each small box represents one gene, with blue and red indicating if flies were fertile or sterile upon knockdown.
D) The degree to which a gene may represent a node in a network in each of the classifications was measured by number of physical interactors. Interaction data from BIOGRID was used for this analysis.
E) Components of the Drosophila sumoylation pathway, the Nonspecific Lethal Complex, and proteins involved in nuclear export are primary hits that validate in vivo. WAH could not be validated in vivo because no RNAi fly was available at the time of submission (red asterisk). The text coloring of each gene indicates the result of the validation screen and is consistent with the categories in panel A.
See also Figure S2.