Fig 7.
IFITM proteins do not modulate replication kinetics of RVFV. Equal numbers of cells (3 × 104 cells/well in a 12-well plate) of the Vero E6 cell line stably expressing IFITM-1, -2, or -3 or empty vector were seeded overnight. Cells were incubated with virus at an MOI of 0.5 (solid lines) or 1 (dotted lines) for 1 h and washed to remove unbound virus, and the infection was allowed to proceed for the indicated times. Cells were stained with NP antibody, following which the images were acquired and the percentage of cells that stained positive for NP expression was determined. Data are presented as the percentage of infected cells, are representative of three independent experiments, and are the means ± standard deviations.