Fig 1.
IFITM-2 and IFITM-3 proteins restrict RVFV infection. (A) Western blot analysis of cells of Vero E6 cell lines stably expressing Myc-tagged IFITM-1, IFITM-2, or IFITM-3 or vector alone. (B to F) Vero E6 cells stably expressing IFITM-1, -2, or -3 were infected with RVFV MP-12 (B and C), RVFV ZH501 (D and E), or JUNV (F) at a specific MOI and for a specific duration of time, as indicated. The percentage of infected cells was determined by evaluating the number of cells that expressed the viral protein GP for RVFV MP-12 infections (B and C), NP for RVFV ZH501 infections (D), or NP for JUNV infections (F). Alternatively, the virus yields in culture medium samples harvested at 24 h postinfection with RVFV ZH501 were determined by plaque assay (E). All data (B to F) are expressed as means ± standard deviations. (G) Representative images of Vero E6 cells stably expressing IFITM-1, IFITM-2, or IFITM-3 or the vector alone that were infected with RVFV MP-12 at an MOI of 0.01 for 36 h and stained to detect the expression of GP (red) and nuclei (blue).