Fig. 1.
Oncogenic Myc activity regulates mTOR-dependent phosphorylation of 4EBP1 at the earliest stages of tumorigenesis to promote cell survival. (A) Representative Western blot of mTOR signaling in wild-type B cells and Eµ-Myc pretumor cells from 4-wk-old mice and quantification of phosphoprotein levels relative to total protein levels in these cells (quantification is for four wild-type and four Eµ-Myc mice). Graph represents mean ± SD. (B) Cell cycle analysis of pretumor cells isolated from Eµ-Myc mice treated with vehicle, RAD001, or MLN0128 for 3 d (three to four mice per arm). (C) Analysis of apoptosis in pretumor cells isolated from Eµ-Myc mice treated with vehicle, RAD001, or MLN0128 (two mice per arm, representative of two experiments). Graph represents mean ± SD.