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Mycobacterium chimaera strain typing using randomly amplified polymorphic DNA (RAPD)-PCR. Shown are RAPD-PCR patterns of M. chimaera clinical isolates from the two patients (lane 1, patient 1; lane 2, patient 2) and of eight respiratory culture isolates from eight different patients (lanes 3 to 10). RAPD-PCR patterns were generated with primers IS986-FP (A) and OPA18 (B). MW, molecular weight marker.
