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. 2013 Jun 14;62(7):2500–2508. doi: 10.2337/db12-1726

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© 2013 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 2. — sCD127 inhibits IL-7 signaling and IL-7–mediated T-cell proliferation. A: Representative fluorescence-activated cell sorting (FACS) plot showing pSTAT5 and pAkt of gated CD4⁺ T cells from PBMCs cultured with IL-7 (1 ng/mL) in the absence or presence of sCD127 (100 ng/mL). B: MFI (y-axis) of pSTAT5 and pAkt in CD4⁺ and CD8⁺ T cells from PBMCs from healthy subjects and patients with T1D cultured with medium, IL-7 (1 ng/mL), and IL-7 + sCD127 (100 ng/mL); n = 6 samples. C: Representative FACS plot showing proliferation of gated CD4⁺ T cells from CFSE-labeled PBMCs cultured for 5 days with GAD65 (5 μg/mL) and IL-7 (1 ng/mL) in the presence or absence of sCD127 (100 ng/mL). D: Proliferation of gated CD4⁺ T cells (%CD4⁺CFSE^dim, y-axis) from healthy subjects and patients with T1D stimulated with medium, GAD65 (5 mg/mL), TT (40LFU), and anti-CD3/CD28–coated beads (αIFN; 1:100 bead/T cell ratio) in medium, IL-7 (1 ng/mL), and IL-7 + sCD127 (100 ng/mL); n = 9 samples. *P < 0.05, **P < 0.01, ***P < 0.001. Lines in B and D represent means.