Figure 5.
IL-13 signals through IL-13R decreases in ROR-γT relative expression and increases in STAT6 phosphorylation and GATA3 relative expression. (A) ROR-γT mRNA expression with each sample normalized to GAPDH and relative expression compared to Th17 cells polarized without IL-13 present. (B) Total protein was harvested 3 days after polarization and phospho-STAT6 and total STAT6 levels were determined. Blot is representative of 3 separate experiments with densitometry is shown graphically below blot. (A) GATA3 mRNA expression with each sample normalized to GAPDH and relative expression compared to Th17 cells polarized without IL-13 present. Data is compiled from three different experiments, n=3–5, * p<0.05 compared to Th17 cells (no IL-13), ANOVA.