Figure 1.
IL-13Rα1 expression is increased in Th17 polarized T cells. Total RNA or protein was isolated from naïve (Th0), Th1, Th2, or Th17 cells 4 days after polarization. (A) Real-time PCR was performed for IL-13Rα1. (B) Western blot for IL-13Rα1 (blot is representative of 3 individual blots). (C) Real-time PCR was performed for IL-4Rα and the common γ chain mRNA expression. (D) IL-13Rα1 mRNA expression in Th17 cells polarized in the presence of IL-13. For all real-time PCR experiments, data is normalized to GAPDH and relative expression is compared to Th0 cells. Data is compiled from 3 experiments for panels A, C, and D; n=5, * p < 0.05 compared to Th0 cells in (A) and (C) and compared to Th17 cells (no IL-13) in (D), ANOVA.