Figure 2.
Axonal mRNA localization through heterologous 3′UTR. FRAP analysis of neurons transfected with GFPmyr + 3′ UTR of amphoterin mRNA is shown. A, Representative time-lapse sequences for prebleach, bleach, and postbleach at indicated intervals for control and anisomycin-treated cultures. The boxed region shows the bleached ROI. GFP intensity is shown as a spectrum as indicated. B, Quantitation of average signal intensity ± SEM for the bleached ROI from multiple time-lapse sequences normalized to prebleach intensity. The GFPmyr3′Amph shows a protracted recovery from photobleaching that is attenuated by pretreatment with the protein synthesis inhibitor (*p ≤ 0.05, **p ≤ 0.01 for indicated time point pairs by two-way ANOVA comparing to t = 0 min). Scale bar, 50 μm.