TABLE 3.
Validation of array data by real-time PCR
| Gene for: | Microarray data for:
|
RT-PCR data for:
|
||||
|---|---|---|---|---|---|---|
| PRV
|
HSV
|
PRV
|
HSV
|
|||
| Maximum change (n-fold) | Time (hpi) | Maximum change (n-fold) | Time (hpi) | Change (n-fold) | Change (n-fold) | |
| Amd1a | —a | — | 5.13 | 12 | NDb | −1.3 |
| Arc | 6.41 | 6 | 7.98 | 12 | 31.65 | 4.16 |
| Btg2 | 4.88 | 6 | 4.63 | 12 | 6.06 | −2.35 |
| Casp-3 | −7.88 | 12 | — | — | −1.65 | ND |
| Cox-2 | 11.21 | 8 | 3.41 | 12 | 60.7 | ND |
| Egr-1 | 9.38 | 8 | — | — | 13.8 | ND |
| Gene33 | 41.49 | 8 | — | — | 11.1 | ND |
| HO-1 | 4.06 | 8 | — | — | 2 | ND |
| IL-15 | −3.83 | 12 | — | — | −10.5 | ND |
| Lnk-1 | 4.06 | 8 | — | — | 7.5 | ND |
| MKP-3 | 10.92 | 8 | — | — | 4 | ND |
| Odc-1 | — | — | 14.81 | 12 | ND | −1.8 |
| PRG-1 | 3.18 | 8 | — | — | −1.7 | ND |
| SGK | 5.93 | 8 | — | — | 15.2 | ND |
| TagE4 | 3.25 | 8 | — | — | 3.7 | ND |
a
—, Not changed or absent.
b
ND, not done.