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. Author manuscript; available in PMC: 2013 Jul 9.
Published in final edited form as: Science. 2011 Nov 10;334(6061):1420–1424. doi: 10.1126/science.1213214

Fig. 3. FACS and gene expression of Hopx descendants.

Fig. 3

(A and B) FACS sorted Hopx descendants after a pulse of tamoxifen (HopxERCre/+;R26mT-mG/+). Representative plots from one of three experiments are shown. Analysis of Hopx descendants at the indicated time periods after a single pulse of tamoxifen to HopxERCre/+;R26mT-mG/+ mice demonstrates a gradual increase in the number of cells expressing GFP (orange), representing Hopx derivatives, and a concomitant loss of the tdTomato signal (blue, ubiquitously expressed until inactivated by HopxERCre/+ expression). Percentages of cells expressing GFP are shown in the gated population in (B). (C) Gene expression of GFP-positive cells was determined by qRT-PCR (normalized to GAPDH). Results are expressed relative to the level of gene expression observed 18 hours after tamoxifen induction, n=3. (D) Rate of growth per organoid, n=5. Hopx derivatives 18 h (blue) or 4 d (brown) after tamoxifen induction of HopxERCre/+;R26mT-mG/+ mice, and Lgr5hi (green) cells from HopxLacZ/+;Lgr5EGFP-ERCre/+ mice were utilized (error bars: 1 s.d.).