Skip to main content
. Author manuscript; available in PMC: 2014 Jul 3.
Published in final edited form as: Cell Stem Cell. 2013 May 16;13(1):48–61. doi: 10.1016/j.stem.2013.04.012

Figure 2. Pygo2 loss facilitates luminal/alveolar differentiation of MaSC/basal cells in 3D-Matrigel culture.

Figure 2

(A) Experimental procedure. (B) Examples of branched (b) and acinar-like (a) colonies produced after differentiation induction with HIP (see Experimental Procedures for details). Patterns of K8 (red) and K14 (green) expression as analyzed by confocal microscopy are shown in 3D reconstructed (left) and single plane (right) images. Arrowheads indicate a few K14-positive basal cells bordering an acinar colony. (C) Morphology (left) and quantification (right) of colonies produced by control and SSKO (n=3 each) MaSC/basal cells 6 (left), 4 and 8 (right) days after differentiation induction. (D) RT-qPCR analysis of expression of the indicated genes in control and SSKO (n=3 each) colonies produced 6 days after HIP induction. (E) Genotyping analysis of uninfected or Ade-Cre-infected Pygo2flox/+ and Pygo2flox/flox MaSC/basal cells. (F) RT-qPCR analysis of Pygo2 expression in cells 3 days after infection. (G) Effect of acute Pygo2 deletion on colony formation. Shown are results of quantitative analysis of Ade-Cre-infected MaSC/basal cells from 4 pairs of Pygo2flox/+ and Pygo2flox/flox mice. Values are means ± SD; * p<0.05; ** p<0.01. Bar = 50 µm in (B) and 300 µm in (C). See also Figure S1.