Fig. 1.
Characterization of pE3-Aβ mAb07/1. a Western blot of Aβ(1–40), Aβ(3–40) and pE3-Aβ(3–40), detected with mAb07/1 or mAb6E10. Peptides (20 ng each) were separated in gels containing 8 M urea. The anti-pE3-Aβ mAb does not detect the truncated precursor or full-length Aβ. b Analysis of antibody binding using surface plasmon resonance. The peptides were covalently linked and the antibody applied in a buffered solution. Significant binding of mAb07/1 was only observed with pE3-Aβ (3–40) immobilized on the surface. Other peptides analyzed are: MCP-1 and 2; gastrin; GnRH; neurotensin; orexin; TRH; the N-terminus of collagen, and fibronectin.