Fig. 2.
Quantitative framework for quantitative phenotypes from pooled screens. (A) Illustration of exponential growth of WT cells (black) and cells expressing an shRNA X (orange) under standard conditions (unselected population, solid lines) or selective pressure (selected population, dashed lines). shRNA X affects the growth rate g for unselected cells by a factor (1 + γX) and the selective pressure k by a factor (1 – ρX). (B) Illustration of the biological meaning of the quantitative phenotypes γ and ρ. (C–E) Agreement between shRNA phenotypes. Gray lines: WT phenotype. (C) K562 cell growth γs from two independent experimental replicates. (D) K562 cell ricin resistance ρs determined at different time points of the same experiment. (E) K562 cell ricin resistance ρs from experiments with slightly different doses of ricin.