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. Author manuscript; available in PMC: 2013 Jun 24.
Published in final edited form as: Vaccine. 2007 Mar 22;25(21):4183–4192. doi: 10.1016/j.vaccine.2007.03.008

Figure 3. Expression of functional anti-CEA scFv on the surface of VNP/GS18IND.

Figure 3

(A) FACS analysis of CEA-FITC binding to VNP/18GSIND. Bacteria were incubated with medium alone (control, dotted line) or with FITC-conjugated CEA (solid line), or pretreated for 15 minutes with unlabeled CEA and incubated with FITC-conjugated CEA (dashed line). Data are representative of three independent experiments. (B) Confocal images of VNP/GS18IND stained with CEA-Alexa-Fluor (left panel), CMTMR (central panel). The right panel represents the overlay of both stainings. Data are representative of two experiments.