Figure 6.
PKA inhibits Yki in Drosophila. (A) In Drosophila S2R+ cells, knockdown of PKA-C1 by RNAi increased Yki/Sd reporter activity. (B) Relative transcript levels of ex, CycE, and Diap1 genes in wild-type (blue), C5-Gal4/UAS-PKA-C1 RNAi (red), and C5-Gal4/UAS-PKA-C1 (green) larval wing discs. (C) Yki phosphorylation was increased in C5-Gal4/UAS-PKA-C1 larval wing discs. (D–F) en-Gal4/UAS-PKA-C1 UAS-GFP larval wing discs exhibiting expression of GFP marker (D, green), Diap1 protein (E, red), and Caspase3 (F, white). (G) Merge of D–F.