FIGURE 4:
Genetic analysis of Skb1 with Pom1 and Nif1. (A) Length of dividing, septated cells of the indicated genotypes (mean ± SD; n > 50 for each value). Measurements were performed in the elongated cdc25-22 strain background to increase sensitivity. (B) Growth assay for indicated strains. Tenfold serial dilutions of cells from the indicated genotypes grown at 25 and 34°C. Note that skb1∆ and nif1∆ exhibit additive suppression of cdc25-22 temperature sensitivity, consistent with cell length measurements. (C) Schematic of genetic pathway for Skb1. Proteins in red are mitotic inhibitors and those in green are mitotic inducers. Question mark denotes unclear role for Pom1 in Skb1 function.
