Figure 7.
AQP4 expression in the developing glial scar. AQP4 expression was evaluated within control cortex (A) and within the developing cortical glial scar after moderate TBI (C–H). (B) Overall, AQP4 expression was quantified at 3, 7, and 14 days post traumatic brain injury (TBI). ***P<0.001 vs. control (one-way ANOVA). (C, E, G) Representative confocal images depict glial fibrillary acidic protein (GFAP) (red) and AQP4 (green) co-localization in the evolving glial scar. (D, F, H) Isolation of the AQP4 emission channel demonstrates dramatic perturbation of AQP4 localization in the evolving glial scar. Scale bars: 25 μm.