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. 2013 Jun 3;210(6):1235–1249. doi: 10.1084/jem.20130221

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© 2013 Diskin et al.

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Figure 8. — Analysis of the potential N-linked glycosylation site at Asn279_gp120. (A) Sequence alignment of YU-2, the two YU-2 Ala281_gp120 mutants, and the three known HIV strains with a potential N-linked glycosylation site at Asn279_gp120. The glycosylation potential for Asn279_gp120 was calculated for each strain using NetNGlyc 1.0 Server. A glycosylation site is predicted (red highlighted numbers) if the glycosylation potential is ≥0.5. (B) Replication profiles of YU-2 escape mutants. Two independent experiments compared the replication of various YU-2 escape mutants to YU-2 WT in PBMC cell culture. Levels of virus in the supernatant were determined by measuring p24 levels at various time points after inoculation, and each value represents the mean of two replicates each from two independent experiments. (C) Neutralization curves for 45-46m2 and 45-46m7 against YU-2^A281T and YU-2^A281S. The curves for YU-2^A281T were derived using an extended concentration series. Error bars represent standard deviation from the mean. (D) Neutralization of A281T-associated mutations affecting the Asn276_gp120-linked glycan.