FIGURE 2.
Interleukin (IL)-1β messenger RNA (mRNA) expression in platelets. mRNA was collected from freshly isolated, unactivated platelets and processed as recently described. This figure is a visualization using the Integrated Genome Browser (IGB) of paired-end next-generation RNA-sequencing (next-gen RNA-seq) reads that are aligned to genes for secreted protein acidic and rich in cysteine (SPARC, top panel) or IL-1β (bottom panel) (genes are in a 3′ to 5′ orientation). Gene maps (bottom portion of each figure) are represented by thick (exons) and thin (introns) horizontal lines. The bars immediately above the gene represent sequencing reads from platelet transcripts that were fragmented, sequenced, and aligned to IL-1β. The lines at the top represent paired-end sequence reads that align within exons only, introns only, or span exon/exon or exon/intron junctions. As shown, exon/exon junction spanning sequences are common for SPARC and rare for IL-1β, whereas intronic and intron/exon junction spanning reads are common for IL-1β and rare for SPARC.