Figure 4.
Identification of specific and cross-reactive TCR clones
T hybridoma cell populations of clonally expanded CDR3α mutants were incubated for 24h with T2 A2Kb cells and A. varying indicated concentrations of gp209-2M, B. no peptide (top); irrelevant Mart 1 (27L) (10 μM) peptide (middle) or no peptide and no T2 A2Kb cells (bottom). Secreted IL-2 was quantified using IL-2 ELISA. Specific clones (S) and cross-reactive clones (C) are defined as those that do not and do respectively, secrete IL-2 in the absence of relevant peptide, respectively. Error bars represent standard deviation of triplicate repeats. Experiments were repeated twice.