Figure 6. IFN-γ and perforin are involved in chNKG2D T cell-mediated anti-angiogenesis in vivo.
(A) Tumor-bearing mice that were inoculated s.c. with 5×105 B16F10 cells on day 0 were treated with two doses of chNKG2D T cells (2×106) derived from either WT B6 (■), IFN-γ−/−(▲) and Pfp−/−(•) mice on days 7 and 9. WtNKG2D transduced T cells (◇) from B6 mice were used as negative controls. Tumor diameters were measured every 2 days starting 7 days post-tumor injection. The results shown are pooled data from 2 independent experiments. The tumor areas are represented as Mean + SEM. (B) Representative images of tumor-inoculated sites in T cell–treated mice on day 15. (C) Tumor-inoculated sites were isolated from T cell–treated mice at day 15 and tumor-supplying vessels were counted. Data are shown as the individual values of 8 mice in each group, pooled from 2 independent experiments.