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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Nat Methods. 2013 Mar 24;10(5):421–426. doi: 10.1038/nmeth.2411

Figure 2.

Figure 2

Characterization of in vivo transcription factor diffusion. (a) Examples of single molecule tracking of a fast-diffusing mEos2-GR molecule (upper panel) and of a DNA-bound molecule (lower panel) in presence of 100 nM dexamethasone at 10 ms time resolution. (b) Cumulative distribution functions of squared displacements of mEos2-H4 and mEos2-GR without or with 100 nM dexamethasone treatment (n = 3336, 7 cells (GRd), n = 1644, 4 cells (GRu), n = 2020, 8 cells (H4)). Black lines indicate fits with three effective diffusion components to the distributions (Equation 2 in online methods). Inset: fractions of molecules exhibiting slow effective diffusion corresponding to DNA-bound fraction (dark blue) and molecules exhibiting fast effective diffusion (light blue).