FIGURE 2.
Establishment of IL-22–producing Th22 cells from uveitis patients with Behçet’s disease. (A) Purified CD4+ T cells from Behçet’s disease patients (BD; n = 4, black bars) with active uveitis or healthy donors (HD; n = 4, open bars) in the presence of anti-CD3/CD28 Abs, anti–IFN-γ Ab, anti–IL-4 Ab, rIL-2, rIL-6, and rTNF-α. Supernatants of polarized Th22 cell lines were harvested for ELISA analysis (ng/ml). (B) For flow cytometric analysis, harvested Th22 cell lines from patients with Behçet’s disease (n = 2) or a healthy donor (n = 1) were stained with anti-CCR10 Abs and anti-CD4 Abs. The percentages of cells that were double positive for CCR10/CD4 are shown.