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. Author manuscript; available in PMC: 2013 May 21.
Published in final edited form as: Cancer Res. 2011 Dec 20;72(4):897–907. doi: 10.1158/0008-5472.CAN-11-2681

Figure 3. Enhanced Hedgehog signaling due to Ptch1 heterozygosity increased OC function in a cell-autonomous manner.

Figure 3

A) Serum CTX was increased in Ptch1+/− mice compared to WT littermates (n=7/group). B) Increased actin ring formation (top) and bone resorption lacunae (bottom) in Ptch1+/− OC differentiated on bovine bone slices for 6 days. C) Quantification of resorption lacunae area. D) Proliferation of Ptch1+/− and WT macrophages in response to 100 ng/mL MCSF by BrdU incorporation assay. E) Immunoblotting of p-AKT substrates and β-actin of Ptch1+/− and WT day 3 pre-OC starved for 1 hour and stimulated with 50 ng/mL MCSF or 50 ng/mL RANKL for 30 minutes.