Figure 2.
Acute EtOH inhibits stimulus-evoked NMDA but not AMPA EPSCs in lOFC neurons. (a) Time-course effects of EtOH on amplitude of evoked NMDA and AMPA EPSCs. Data shown are percent of pre-EtOH baseline. Representative traces show NMDA currents recorded at +40 mV in the absence and presence of 66 mM EtOH. (b) Summary chart shows amplitude of NMDA EPSCs during the last 2 min of each recording epoch (baseline, treatment, washout) for 11, 33, and 66 mM EtOH. Only the highest EtOH concentration (66 mM) inhibited NMDA EPSC amplitude. Symbols: *=differs from baseline. #=differs from baseline, 11, 33 mM EtOH. (c) Time-course effects of the NR2B-NMDA receptor antagonist RO 25-6981 and EtOH on amplitudes of evoked NMDA EPSCs. Example trace shows effects of RO 25-6981 alone and in combination with EtOH on evoked NMDA EPSCs. (d) Summary chart shows amplitude (as percent of pre-drug control) for evoked NMDA EPSCs during the last 2 min of each recording epoch (baseline, RO 25-6981, RO 25-6981+EtOH). Symbols: *=differs from baseline. #=differs from RO 25-6981. (e) EtOH (66 mM) has no effect on amplitude of evoked AMPA EPSCs. Data shown are percent of pre-EtOH baseline. Representative traces show AMPA currents in the absence (blue) and presence (red) of 66 mM EtOH. All bars and data points represent mean ±SEM. N=4–7 for each treatment group. A color reproduction of this figure is available on the Neuropsychopharmacology journal online.