Figure 5. Correlation of responses against PD-L1-derived long and short peptides. (A) Correlation of cells releasing IFNγ in response to PDLong1 or the HLA-A2-restricted, PD-L1-derived peptide PDL115–23 (PD-L101). PBMCs from HLA-A2+ individuals were stimulated with PDL101 once in vitro before being plated at 5 × 105 cells per well either in the absence or in the presence of PDL101. A Spearman correlation test confirmed a significant correlation between T-cell reactivity toward short and long PD-L1-derived epitopes (p = 0.03). (B) Correlation of cells releasing TNFα in response to PDLong1 or PD-L101. PBMCs from HLA-A2 positive individuals were stimulated with PDL101 once in vitro before being plated at 5 × 105 cells per well either in the absence or in the presence of PDL101. A Spearman correlation test indicates that there is no correlation between T-cell reactivity toward short and long PD-L1-derived epitopes (p = 0.74).