Table 1.
qRT-PCR assessment of gene transcription in MSCs challenged with LPS.
| LPS | IL1A | IL1β | IL6 | IL8 | iNOS |
|---|---|---|---|---|---|
| ng/mL | Expression, a.u. | Expression, a.u. | Expression, a.u. | Expression, a.u. | Expression, a.u. |
| 0 | 1 ± 3.25 | 1 ± 1.82 | 1 ± 1.54 | 1 ± 1.41 | 1 ± 3.14 |
| 50 | 194 ± 2.77* | 317 ± 1.63* | 99 ± 1.48* | 26 ± 1.8* | 882 ± 2.46* |
| 100 | 265 ± 2.33* | 465 ± 1.78* | 155 ± 1.4* | 46 ± 1.36* | 1128 ± 2.3* |
| 500 | 315 ± 2.35* | 755 ± 1.68* | 47 ± 2.67* | 18 ± 1.98* | 1596 ± 2.46* |
| 1000 | 247 ± 2.38* | 498 ± 1.78* | 98 ± 1.6* | 11 ± 1.61* | 1489 ± 2.37* |
| 2500 | 338 ± 2.35* | 729 ± 1.57* | 224 ± 2.32* | 20 ± 3.33* | 1438 ± 2.25* |
Conditions: MSCs were incubated with 50 ng/mL–2500 ng/mL LPS for 3 h and then were lysed for mRNA extraction and qRT-PCR analysis. The presented data are statistically significant at the confidence level *P < 0.005, (n = 3).