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. 1987 Jul;7(7):2592–2596. doi: 10.1128/mcb.7.7.2592-2596.1987

Structural and functional characterization of the promoter region of the mouse c-Ki-ras gene.

E K Hoffman, S P Trusko, N Freeman, D L George
PMCID: PMC365395  PMID: 3614201

Abstract

We isolated and characterized the 5' region of the mouse c-Ki-ras gene, including a 5' untranslated exon (exon 0). These studies used genetic material from Y1 mouse adrenocortical tumor cells in which the c-Ki-ras gene is amplified and overexpressed. Our data demonstrate that transcription initiates at multiple sites, predicting size heterogeneity at the 5' ends of the c-Ki-ras mRNAs. Using transient expression assays, we identified a genomic fragment within the 5' region which exhibits bidirectional promoter activity.

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Selected References

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