Fig. 5.
Gas6 stimulate Vav phosphorylation in microglia. a BV-2 cells and THP-1 cells were treated with Gas6 (10 nM) for the indicated periods of time. Vav phosphorylation was evaluated by immunoprecipitation with an anti-Vav antibody followed by Western blot analysis using the anti-phosphotyrosine antibody 4G10. Blots were then stripped and reprobed with an anti-Vav antibody as a protein-loading control. b Analysis of band intensity of Western blots of phosphorylated Vav normalized to Vav protein levels and expressed as relative ratio. The data which represent the results of five independent experiments (THP-1) revealed a significant upregulation of Vav phosphorylation at 5 and 10 min after Gas6 stimulation. c Primary microglia from wild-type or vav−/− mice were incubated with vehicle or Gas6 (10 nM) for 30 min, and phagocytosis was quantified by counting the uptake of fluorescent microspheres. The data are pooled from six independent experiments. ***p<0.0001; *p<0.01 compared with control