FIGURE 6.
gp120 regulates the Wnt5a/CaMKII and Wnt5a/JNK signaling pathways in the SDH. A and B, time courses of pT286-αCaMKII (A) and pT183/Y185-JNK (B) proteins after i.t. gp120 (100 ng). C and D, inhibition of Wnt5a signaling by Box5 blocked i.t. gp120-induced pT286-αCaMKII (C) and pT183/Y185-JNK (D) increase. E and F, effect of sFRP3 on gp120-induced pT286-αCaMKII (E) and pT183/Y185-JNK (F) increase. Box5 (10 μg), sFRP3 (2 μg) or vehicle was intrathecally administered 30 min prior to gp120 (100 ng) or vehicle. The SDH was collected at 12 h after gp120 injection. In summary graphs, the levels of target proteins were normalized with the β-actin loading control and expressed as relative units to the control (0 h or vehicle). Data presented in graphs are means ± S.E. from at least 3 mice. *, p < 0.05; **, p < 0.01; #, p < 0.05 (one way ANOVA).