Figure 3. Silencing let-7e in vivo ameliorates the development of EAE.
(A) Lentivirus vectors overexpress (LV-pre) or inhibit (LV-ctrl) let-7e expression. The sequence of specific inhibitor of miR-let-7e is shown. (B) WT mice were injected intravenously with 30 × 106 lentivirus 7 days before MOG35-55 immunization; (C)splenocytes from virus-infected EAE mice on day 8 were restimulated with MOG35-55 for 3 days; CD4+ T cells were then isolated and transferred into naïve WT mice; the mean clinical scores were presented. (D) The expression of let-7e in the transferred cells was detected by QPCR. *: p<0.02, **: p<0.01, ***: p<0.007, versus LV-ctrl. (B and C) The mean clinical scores is shown (n = 10, Mann-Whitney test). (D) Data are shown as mean ± SEM of n = 5 (ANOVA test) and (B-D) are representative of three independent experiments.