Fig 2.
miR-33a and -a* and miR-33b and -b* are coexpressed with their host genes, Srebp-2 and Srebp-1. (A) Quantitative real-time fluorescence PCR (qRT-PCR) analysis of miR-33a, miR-33a*, and SREBP-2 in human hepatic (Huh7) cells loaded with cholesterol by nLDL (120 μg/ml) and depleted of cholesterol by statin treatment (5 μM). (B) qRT-PCR analysis of miR-33b, miR-33b*, and SREBP-1c in Huh7 cells treated with the LXR agonist T0901317 (T090) for 12 h. (C) qRT-PCR analysis of SREBP-2 and miR-33a and miR-33a* in the livers of C57BL6 mice (n = 5 per group) fed a chow diet, high-fat diet (HFD), or rosuvastatin-supplemented diet (statin). (D) qRT-PCR analysis of SREBP-1c, miR-33b, and miR-33b* in the livers of rhesus monkeys (n = 6 per treatment) after 1 h of glucose stimulation. In panels A to D, the data are means ± SEM and representative of ≥2 experiments in triplicate. *, P ≤ 0.05.